منابع مشابه
Immunity to K1 Killer Toxin Internal TOK1 Blockade
K1 killer strains of Saccharomyces cerevisiae harbor RNA viruses that mediate secretion of K1, a protein toxin that kills virus-free cells. Recently, external K1 toxin was shown to directly activate TOK1 channels in the plasma membranes of sensitive yeast cells, leading to excess potassium flux and cell death. Here, a mechanism by which killer cells resist their own toxin is shown: internal tox...
متن کاملA Saccharomyces cerevisiae genome-wide mutant screen for altered sensitivity to K1 killer toxin.
Using the set of Saccharomyces cerevisiae mutants individually deleted for 5718 yeast genes, we screened for altered sensitivity to the antifungal protein, K1 killer toxin, that binds to a cell wall beta-glucan receptor and subsequently forms lethal pores in the plasma membrane. Mutations in 268 genes, including 42 in genes of unknown function, had a phenotype, often mild, with 186 showing resi...
متن کاملExpression of K1 Toxin Derivatives in Saccharomyces cerevisiae Mimics Treatment with Exogenous Toxin and Provides a Useful Tool for Elucidating K1 Mechanisms of Action and Immunity
Killer toxin K1 is a heterodimeric protein toxin secreted by Saccharomyces cerevisiae strains infected with the M1 double-stranded RNA 'killer' virus. After binding to a primary receptor at the level of the cell wall, K1 interacts with its secondary plasma membrane receptor Kre1p, eventually leading to an ionophoric disruption of membrane function. Although it has been under investigation for d...
متن کاملDissecting toxin immunity in virus-infected killer yeast uncovers an intrinsic strategy of self-protection.
Toxin-secreting "killer" yeasts were initially identified >40 years ago in Saccharomyces cerevisiae strains infected with a double-stranded RNA "killer" virus. Despite extensive research conducted on yeast killer toxins, the mechanism of protecting immunity by which toxin-producing cells evade the lethal activities of these proteins has remained elusive. Here, we identify the mechanism leading ...
متن کاملComparison of Anti-cryptococcal Killer Toxin
s: Keszthelyi A Characterisation of anticryptococcal FC-1 toxin of Filobasidium capsuligenum, Acta Biologica Szegediensis Volume 50(3-4):155, 2006 Posters: 35th Annual Conference on Yeasts, 2007, Smolenice, Slovakia, Poster: Andrea Keszthelyi, Zoltan Farkas, Zsuzsanna Hamari, Ilona Pfeiffer, Csaba Vágvölgyi, Judit Kucsera Comparison of killer toxin producing and toxin non-producing Filobasidium...
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ژورنال
عنوان ژورنال: Cell
سال: 2001
ISSN: 0092-8674
DOI: 10.1016/s0092-8674(01)00376-2